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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Potato Virus Y-resistant Potato
EN
SY233
Yes
TIC-AR233-5
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Organization:Tecnoplant SA ()Tecnoplant SA Constitución 4234 (1254) Capital FederalBuenos Aires, Capital Federal
, ArgentinaPhone: +54 11 4909 8138,Fax: +54 11 4909 8055,Email:
The potato was modified for resistance to Potato Virus Y infection. The modified potato expresses the viral coat protein to cross-protect the plant from viral inoculation, spread, multiplication and symptom expression. Although the mechanism is not well-defined, likely the production of the coat protein enhances the production of movement proteins and coordinates the formation of viral replication complexes, thus limiting the spread of the viral via cell-to-cell infections. Additionally, the modified potato expresses neomycin phosphotransferase II for kanamycin selection during transformation.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
EN
pBI121
EN
- Agrobacterium-mediated DNA transfer
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0.180 kb
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0.795 kb
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0.250 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-15020-3 PVY coat protein | Potato virus Y (PVY)Protein coding sequence | Resistance to diseases and pests (Viruses, Potato virus Y (PVY))
The modified potato (Solanum tuberosum) contains two genetic cassettes for expression of Escherichia coli neomycin phosphotransferase II (nptII) and Potato Virus Y (PVY) coat protein.
Transcription of nptII begins at an Agrobacterium tumefaciens nopaline synthase (nos) promoter and terminates at an A. tumefaciens nos terminator. Neomycin phosphotransferase is a selectible marker used for selecting transformants.
Transcription of PVY coat protein initiates from a Cauliflower Mosaic Virus 35S promoter and terminates at a A. tumefaciens nos terminator. The sequence of the PVY coat protein contains the last 17 codons of the viral replicase gene, the full coat protein sequence and the 3' noncoding region.
Note:
- Southern blot analysis indicated multiple transgene insertions.
EN
Transcription of nptII begins at an Agrobacterium tumefaciens nopaline synthase (nos) promoter and terminates at an A. tumefaciens nos terminator. Neomycin phosphotransferase is a selectible marker used for selecting transformants.
Transcription of PVY coat protein initiates from a Cauliflower Mosaic Virus 35S promoter and terminates at a A. tumefaciens nos terminator. The sequence of the PVY coat protein contains the last 17 codons of the viral replicase gene, the full coat protein sequence and the 3' noncoding region.
Note:
- Southern blot analysis indicated multiple transgene insertions.
EN
- Food
Western blot analysis indicated that NPTII was present and readily detected from leaf and tuber samples. From leaf extracts, the PVY coat protein was difficult to detect.
PCR analysis confirmed the presence of both nptII and PVY coat protein.
Southern blot analysis indicated three insertions of the PVY coat protein.
Northern blot analysis detected low levels of PVY coat protein transcript in root, stem and leaf tissues. Detection relied on extended autoradiographic exposure.
For more information, kindly refer to the attached article (Bravo-Almonacid et al., 2012)*.
*Note: Methods used in the article are not validated.
EN
PCR analysis confirmed the presence of both nptII and PVY coat protein.
Southern blot analysis indicated three insertions of the PVY coat protein.
Northern blot analysis detected low levels of PVY coat protein transcript in root, stem and leaf tissues. Detection relied on extended autoradiographic exposure.
For more information, kindly refer to the attached article (Bravo-Almonacid et al., 2012)*.
*Note: Methods used in the article are not validated.
EN
